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Chapter 2 Literature Review6
2.1 Diabetes Mellitus6
2.1.1 Type 1 Diabetes Mellitus6
2.1.2 Type 2 Diabetes Mellitus7
2.1.3 Other specific types of DM8
2.1.4 Diabetes Mellitus prevalence and incidence8
2.1.5 Maturity Onset Diabetes of the Young9
2.2 Gestational diabetes mellitus16
2.2.1 Definition16
2.2.2 Prevalence and incidence of GDM17
2.2.3 Risk factors for GDM19
2.2.5 Etiology, Pathophysiology and complications of GDM20
2.2.4 Screening and diagnostic criteria of GDM25
2.2.5 Management, Treatment and Supervision of GDM31
2.5 Glucokinase Gene33
2.5.1 Location GCK gene33
2.5.2 Definition, function and role GCK in glucose stimulated insulin secretion33
2.5.3 Regulation of glucokinase gene expression36
2.5.3 Role Glucokinase (GCK) gene in GDM37
Chapter 3 Material and Methods41
3.1 Study design41
3.2 Study population41
3.3 Study location41
3.4 Inclusion criteria41
3.5 Exclusion criteria41
3.6 Ethical considerations41
3.7 Data collection42
3.7.1 Questionnaire42
3.7.2 Body Max Index (BMI)42
3.7.3 Blood samples collection and processing42
3.8 Materials42
3.8.1 Instruments42
3.8.3 PCR primers44
3.9 Biochemical Analysis45
3.9.1 Determination of Serum Glucose45
3.9.2 Determination of Glycated Hemoglobin45
3.9.3 Determination of Oral glucose tolerance test46
3.9.4 Determination of serum cholesterol46
3.9.5 Determination of serum triglycerides46
3.9.6 Determination of High-Density Lipoprotein cholesterol47
3.9.7 Calculation of Low-Density Lipoprotein cholesterol47
3.8.10 Determination of Homeostasis model assessment-insulin resistance47
3.9.8 Determination of Insulin47
3.10 Molecular analysis48
3.10.1 DNA extraction and purification48
3.10.2 Glucokinase gene mutations screening48
3.10.5 Agarose gel electrophoresis preparation (3.0%)50
3.10.5 Statistical analysis51
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